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. 2022 Nov 30;12:20629. doi: 10.1038/s41598-022-25083-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of one carbon supplementation on (a) MII oocyte rate, (b) Cleavage rate, (c) Blastocyst rate (Blastocyst/Cleaved embryos), (d) Blastocyst rate (Blastocyst/Oocytes) and (e) Embryo cell number. (f) Representative images from differential staining of blastocysts of CMM and OCS groups. Magnification 20 ×; Scale bar, 100 µm. ICM inner cell mass, TE trophectoderm cells, TCN total cell number. The data are shown as mean ± s.e.m. NS stands for non-significant differences (P > 0.05). ***stands for P ≤ 0.001. N stands for number of total oocytes or blastocysts in each group. For embryonic development 12 replications, consisting of 700 oocytes, were done for each group.