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. 2022 Nov 30;12:20629. doi: 10.1038/s41598-022-25083-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of one carbon supplementation on relative mRNA expression of target genes in (A) cumulus cells, (B) matured oocytes and (C) blastocysts. (D) Effect of one carbon supplementation on DNMT3a protein expression in terms of fluorescent intensity in matured oocytes. (E) Representative images of DNMT3a immunocytochemistry in matured oocytes (20 × magnification; Scale bar, 100 µm). The data are shown as mean ± s.e.m. NS stands for non-significant differences (P > 0.05). *Stands for P < 0.05. ***Stands for P ≤ 0.001. N stands for total number of oocytes in each group.