Fig. 2.

Sdc4-Tg mice show exacerbated cardiac remodelling upon pressure overload. A Representative magnetic resonance imaging (MRI), mid-ventricular, short-axis view, of Sdc4-Tg and wild-type (WT) mice subjected to aortic banding (AB) or sham operation for 20 weeks (20w). See Tables 2 and SI for characteristics post-AB or sham at 2, 6, 12 and 20w. Relative mRNA levels of signature molecules of heart failure, Nppa and Nppb [B, C; encoding atrial and brain natriuretic peptides (ANP and BNP, respectively)] in left ventricle (LV), right ventricle (RV) and atria of Sdc4-Tg and WT at 20w post-AB or sham (n = 7–9). Relative mRNA levels of markers of pathological cardiomyocyte remodelling, Myh6 and Myh7 [D, E; encoding α- and β-myosin heavy chain (MHC), respectively] in the LV of Sdc4-Tg and WT mice after 20w of AB or sham. Gene expression was normalized to Gapdh. Data are mean ± SEM. Statistical differences were tested using one-way ANOVA with Dunnett’s post-testing vs. respective WT sham, *p < 0.05, **p < 0.01, ***p < 0.001; or vs. WT AB20w, ^#p < 0.05