Table 2.

Performance of Paralog Explorer on a list of 25 curated paralog pairs/triplets from Levo et al (2022).

Paralog 1	Paralog 2	Paralog Explorer Top hit (DIOPT Score)	Note
drm	sob / odd	sob (2), bowl (2)*, odd (1)	bowl is located in same genomic region
slp1	slp2	slp2 (6)	16 additional lower-scoring paralogs (DIOPT <= 5)
H15	mid	mid (9)	7 additional lower-scoring paralogs (DIOPT <= 4)
gcm	gcm2	gcm2 (4)	Only hit
salr	salm	salm (7)	2 additional lower-scoring paralogs (DIOPT = 1)
nub	pdm2	pdm2 (4)	3 additional lower-scoring paralogs (DIOPT <= 3)
dnt	drl	drl (9)	18 additional lower-scoring paralogs (DIOPT <= 6)
inv	en	en (5)	16 additional lower-scoring paralogs (DIOPT = 1)
pyr	ths	none	pyr and ths are reciprocal best BLAST hit (33 % identity)
bab1	bab2	bab2 (5)	23 additional lower-scoring paralogs (DIOPT <= 4)
Doc1	Doc2, Doc3	Doc3 (6), Doc2 (5)	6 additional lower-scoring paralogs (DIOPT <=4)
scyl	chrb	chrb (7)	Only hit
toe	eyg	eyg (4)	22 additional lower-scoring paralogs (DIOPT <=3)
ara	caup	caup (8)	6 additional hits (DIOPT <= 7) including genomically linked mirr
comm	comm2	comm2 (1), comm3* (1)	All 3 genes genomically linked
kni	knrl	knrl (4)	2 additional paralogs (DIOPT <=3)
E5	ems	ems (4)	8 additional paralogs (DIOPT <= 2)
fd96Ca	fd96Cb	fd96Cb (7)	15 additional paralogs (DIOPT <= 4)
dan	danr	danr (3)	1 additional paralog (DIOPT = 1)
ac	sc	l(1)sc* (7), sc (6)	9 additional paralogs (DIOPT <= 5)
Vsx1	Vsx2	Vsx2 (5)	28 additional paralogs (DIOPT = 1)
btd	Sp1	Sp1 (3)	12 additional paralogs (DIOPT <= 3)
NetA	NetB	NetB (6)	8 additional paralogs (DIOPT <= 2)
disco	disco-r	disco-r (8)	1 additional paralog (DIOPT = 1)
B-H2	B-H1	B-H1 (6)	1 additional paralog (DIOPT = 1)

* Additional paralog identified at the same or higher DIOPT score as the predicted hit.