Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 30;13:7366. doi: 10.1038/s41467-022-35166-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Immunofluorescence on sections of the head of a E18.5 Spi1^GFP/+ embryo (PU1-GFP in green), labeled for Lyve1 (red) and CD45 (white) (60 µm maximum intensity projection) shows the presence of the conventional (white arrows) and the non-conventional pvM populations (red arrows) at this stage (n = 3). b No GFP nor Lyve1 fluorescence was observed in the Spi1^GFP/GFP E18.5 dorsal cortex (33 µm maximum intensity projection) (n = 2). c Immunofluorescence on Spi1^GFP/+ adult mouse brain (48 µm maximum intensity projection) (n = 3). d Immunofluorescence on sections of Cx3cr1^GFP/+; Cx3cr1-Cre; Rosa26^tdT mouse brain showing that conventional (white arrows) and non-conventional pvM population (red arrows) are expressing tdTomato (n = 3). e Flow cytometry analysis of Cxcr4-CreErt2; Rosa26^tdT brain parenchyma, gated on living, single cells and CD64⁺ F4/80⁺ CD206⁺ cells. Subsequently histograms of CD45 and Lyve1 expression of the CX3CR1^–tdTomato^– population in blue, the CX3CR1⁺tdTomato⁻ population in red and FMO in gray (n = 3). Source data are provided as a Source Data file.