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. 2022 Nov 30;13:7366. doi: 10.1038/s41467-022-35166-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a–c Cx3cr1^GFP mouse brains were injected intra-ventricular with Dextran-Alexa647 (37 µm maximum intensity projection) (n = 3) and d–f Acetylated-LDL-Alexa594 (42 µm maximum intensity projection) (n = 3). Ten minutes after injection, mice were euthanized and brains dissected. Confocal analysis on brain sections shows Lyve1 (white), Dyes (red), and CX3CR1 (green). The unconventional pvMs phagocytosed the dyes (red arrows), as well as did conventional pvMs (white arrows) (b, c, e, f). g Tiled confocal acquisition of a Prox1^mOrange2 brain section. Lyve1⁺ cells in green lined blood vessels stained for CD31 in blue. Lyve1⁺ cells in green penetrated the brain to the hippocampal fissure (hif) and all the way towards the third ventricle (3 V). (n = 4).