Fig. 11.

Tubular epithelial cell-specific deletion of FFAR4 aggravated kidney damage and cellular senescence in cisplatin-induced AKI mice. a Mating strategy to generate FFAR4 conditional KO in mouse TECs. b, c The sCr and BUN levels in different groups of mice (n = 6; ****P < 0.0001). d Representative images of H&E staining (200×, scale bar = 50 μm; 400×, scale bar = 20 μm). e Tubular injury scores of kidney tissues (n = 6; ****P < 0.0001, ***P < 0.001). f, g Relative mRNA expression of KIM1 and NGAL in kidney tissues (n = 6; ****P < 0.0001, *P < 0.05). h, i Representative images and quantitative analysis of SA-β-gal staining of kidney sections (200×, scale bar = 50 μm; 400×, scale bar = 20 μm; n = 6; ****P < 0.0001). j Protein expression of p53, p21, p-Rb/Rb, LaminB1, ɣH2A.X, and IL-6 in kidney tissues was detected by western blotting and quantified by densitometry (n = 6; ****P < 0.0001, *P < 0.05, FFAR4^fl/fl + CP vs. FFAR4^fl/fl; ^####P < 0.0001, ^##P < 0.01, ^#P < 0.05, FFAR4^tecKO + CP vs. FFAR4^fl/fl + CP). Data are presented as mean ± SD. CP cisplatin