Fig. 6.

Inhibition of FFAR4 aggravated cellular senescence in cisplatin-stimulated renal tubular epithelial cells. a, b TCMK-1 cells were transfected with negative control (NC) siRNA or FFAR4 siRNA for 24 h and then treated with 2 μg/ml cisplatin for 6 h. The knockdown efficiency of FFAR4 siRNA in TCMK-1 cells was evaluated by quantitative real-time PCR analysis and western blot analysis (n = 3; ****P < 0.0001, ^ns no significant). c Protein expression of p53, p21, LaminB1, p-Rb/Rb, ɣH2A.X and IL-6 detected by western blotting and quantified by densitometry (n = 3; ****P < 0.0001, CP + NC siRNA vs. NC siRNA; ^####P < 0.0001, CP + FFAR4 siRNA vs. CP + NC siRNA). d Representative images and quantitative analysis of SA-β-gal staining of renal primary tubular cells (PTCs) from WT and FFAR4-KO mice (100×, scale bar = 250 μm) (n = 3; ****P < 0.0001). Data are presented as mean ± SD. CP cisplatin