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. 2022 Nov 30;7:384. doi: 10.1038/s41392-022-01254-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — FFAR4 suppressing cisplatin-induced senescence via activating SirT3 in AKI mice. a, b Relative mRNA and protein expression of SirT3 in TCMK-1 cells transfected with SirT3 siRNA (n = 3; ****P < 0.001, *P < 0.05, ns. no significant). c Protein expression of p53, p21, LaminB1, p-Rb/Rb, ɣH2A.X and IL-6 in TCMK-1 cells transfected with SirT3 siRNA detected by western blotting and quantified by densitometry (n = 3; ****P < 0.0001, **P < 0.01, CP + NC siRNA vs. NC siRNA; ^$$$$P < 0.0001, CP + TUG891 + NC siRNA vs. CP + NC siRNA; ^####P < 0.0001, ^###P < 0.001, CP + TUG891 + FFAR4 siRNA vs. CP + TUG891 + NC siRNA). d, e Relative mRNA and protein expression of SirT3 in TCMK-1 cells transfected with transfected with empty plasmid (pVector) or plasmid expressing SirT3 (pSirT3) (n = 3; ****P < 0.001, ns no significant). f Protein expression of p53, p21, LaminB1, p-Rb/Rb,ɣH2A.X and IL-6 in TCMK-1 cells transfected with empty plasmid (pVector) or plasmid expressing SirT3 (pSirT3) detected by western blotting and quantified by densitometry (n = 3; ****P < 0.0001, CP + NC siRNA+pVector vs. NC siRNA+ pVector; ^$$$$P < 0.0001, ^$$P < 0.01, CP + FFAR4 siRNA+pVector vs. CP + NC siRNA+pVector; ^####P < 0.0001, CP + FFAR4 siRNA+pSirT3 vs. CP + FFAR4 siRNA+pVector). Data are presented as mean ± SD. CP cisplatin