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. 2022 Nov 30;7:384. doi: 10.1038/s41392-022-01254-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — Activation of FFAR4 reversed the Sirt3 expression via Gq/CaMKKβ/AMPK signaling in cisplatin-stimulated TCMK-1 cells. a, b Relative mRNA and protein expression of Gq in TCMK-1 cells transfected with Gq siRNA (n = 3; *P < 0.05, ****P < 0.0001, ns no significant difference). c Protein expression of SirT3 in TCMK-1 cells transfected with Gq siRNA detected by western blotting and quantified by densitometry (n = 3; ****P < 0.0001, *P < 0.05). d Protein expression of CaMKKβ, p-AMPK/AMPK, and p-ACC1/ACC1 in TCMK-1 cells transfected with Gq siRNA detected by western blotting and quantified by densitometry (n = 3; ****P < 0.0001, CP + NC siRNA vs. NC siRNA; ^$$$$P < 0.0001, CP + TUG891 + NC siRNA vs. CP + NC siRNA; ^####P < 0.0001, CP + TUG891 + Gq siRNA vs. CP + TUG891 + NC siRNA). e Protein expression of p53, p21, p-Rb/Rb, LaminB1, ɣH2A.X and IL-6 in TCMK-1 cells transfected with Gq siRNA detected by western blotting and quantified by densitometry (n = 3; ****P < 0.0001, ***P < 0.001, CP + NC siRNA vs. NC siRNA; ^$$$$P < 0.0001, ^$$P < 0.01, CP + TUG891 + NC siRNA vs. CP + NC siRNA; ^####P < 0.0001, ^#P < 0.05, CP + TUG891 + Gq siRNA vs. CP + TUG891 + NC siRNA^). f TCMK-1 cells were preincubated with 10 μM compound C and TUG891 for 1 h, followed by cisplatin for 6 h. Protein expression of p-AMPK/AMPK and SirT3 detected by western blotting and quantified by densitometry (n = 3; ****P < 0.0001, CP vs. Control; ^$$$$P < 0.0001, CP + TUG891 vs. CP; ^####P < 0.0001, CP + TUG891 + Comp.C vs. CP + TUG891). g Protein expression of p53, p21, p-Rb/Rb, LaminB1, ɣH2A.X and IL-6 detected by western blotting and quantified by densitometry (n = 3; ****P < 0.0001, CP vs. Control; ^$$$$P < 0.0001, CP + TUG891 vs. CP; ^####P < 0.0001, CP + TUG891 + Comp.C vs. CP + TUG891). Data are presented as mean ± SD. CP cisplatin