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. 2022 Nov 30;12:20638. doi: 10.1038/s41598-022-25244-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transplantation of the gut microbiome from healthy Ctrl mice ameliorates bone loss in SCD male recipient mice. μCT analysis of femur from Ctrl and SCD recipient male mice receiving feces from Ctrl or SCD male mice. Three-month-old Ctrl and SCD recipient mice subjected to FMT from Ctrl or SCD donor mice once a week. Six weeks-post FMT, samples were collected. (A) Representative μCT images of distal femur trabecular bone. (B) BV/TV, (C) BV, (D) TV, (E) Tb.N, (F) Tb.Th, and (G) Tb.Sp, of metaphysis. (H) Representative μCT images of mid-diaphysis cortical bone. (I) CtAr/TtAr, (J) Ct.Ar, (K) Tt.Ar, and (L) Ct.Th of mid-diaphysis. n = 6- mice/group. Data are shown as Mean ± SEM with individual data points. Statistical analyses were done using SPSS and p values were calculated using a two-way ANOVA with Tukey’s post hoc analysis. *p < 0.05.