TABLE 1.
Primers used for cloning and site-directed mutagenesis
| Gene | Primer | Nucleotide sequencea |
|---|---|---|
| 85A | 85A left | GAT GAA TTC GCG GAA ATG CCA CCT TCA G |
| 85A right | GAT GGA TCC GCT AGA TGT TGT GTC TGT TCG GAG | |
| 85A left 2 | GATGGATCCA T(1)TT TCC CGG CCG GGC TTG CCG GT G(24) | |
| 85 A right 2 | GAT GAA TTC CTA G(885)GC GCC CTG CGG CGG GCC CGG(865) | |
| 85AL mut 9 | GATGGATCCA T(1)TT TCC CGT CCG GGC TTG CCG GTG GAG(27) | |
| 85AL mut 339 | C(337)GT CAC GTT AAG CCG ACC GGT AGC GCC GTC GTC GGT CTT (375) | |
| 85AR mut 357 | A (357)CC GGT CGG CTT AAC GTG ACG GTT GGC CTG CAG CCA CCC(319) | |
| 85B | 85 B left | GAT GAA TTC ACG ACT TTC GCC CGA ATC GAC ATT TGG |
| 85B right | GAT GGA TCC TCG CGA GGT ATA TCT CAC GTG GAC | |
| 85B left 2 | GATGGATCCA T(1)TC TCC CGG CCG GGG CTG CCG GTC(24) | |
| 85B right 2 | GAT GAA TTC T(852)CA GCC GGC GCC TAA CGA ACT CTG CAG (826) | |
| 85B L mut 9/15 | GATGGATCCA T(1)TC TCC CGT CCG GGT CTG CCG(21) | |
| 85 R 627 mut | A(627)AC CCA CAG ACG CGT GTT GTT TGC GAC CAG CTT GGG GAT CTG(585) | |
| 85 BL613 mut | A(613)CG CGT CTG TGG GTT TAT TGC GGG AAC GGC ACC CC (647) | |
| 85 B probe L | C(16)TGCCGGTCGAGTACCTGCAGGTGCCGTCG (46) | |
| 85B probe R | G(612)TTGTTTGCGACCAGCTTGGGGATCTGCTG(582) | |
| 85C | 85C left | GAT GAA TTC GTT GGG ATT GGT AGT AGC TAT GAC |
| 85 C right | GAT GGA TCC GGC TCA GGC GGC CGG CGC AGC AGG GGC | |
| 85C left 2 | GATGGATCCA T(1)TC TCT AGG CCC GGT CTT CCA GTG(24) | |
| 85C right 2 | GAT GAA TTC TCA G(882)GC GGC CGG CGC AGC AGG GGC(862) | |
| SOD | SOD left | GATGGATCCA G(1)CC GAA TAC ACC TTG CCA GAC(21) |
| SOD right | GATGAATTCCTA G(618)CC GAA TAT CAA CCC CTT GGT(598) | |
| SOD Lmut 192 | G(184)AA AAG AAC CTG GCT TTC AAC CTC GCC GGC(213) | |
| SOD Rmut 192 | G(210)GC GAG GTT GAA AGC CAG GTT CTT TTC GTT CAG CAA(175) | |
| SOD Lmut 414 | C(406)TG CTG ATC TTC CAG GTT TAC GAC(429) | |
| SOD Rmut 414 | G(426)TA AAC CTG GAA GAT CAG CAG CTT(403) |
a
Nucleotides are numbered beginning with the first base in the portion of the gene encoding the mature exoprotein. Bases corresponding to nucleotide substitutions introduced by site-directed mutagenesis are underlined.