TABLE 2.
Number of low-usage codons in individual 85 antigen genes
| Usage in E. colia | Codons | No. of codonsb
|
||
|---|---|---|---|---|
| 85A | 85B | 85C | ||
| Least-used codons (<8%) | AGA, AGG, CGA, CGG, ATA, CTA, CCC, TCG | 23 | 24 | 25 |
| Adjacent least-used codons | 0 | 2 pairs | 0 | |
| 9 to 12% | GGA, CTC, CTT, TTG, TTA, ACA, TCA | 10 | 11 | 18 |
| 13 to 15% | GGG, CCT, TCG | 4 | 12 | 4 |
| Total low-usage codons | 37 | 47 | 47 | |
a
Usage in E. coli taken from work by Zhang et al. (25). Percent values refer to the percent occurrence of the codon relative to its synonymous codon family.
b
Total number of codons within the open reading frame of the exoprotein.