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Relative antigen 85B mRNA concentration in expression systems containing synthetic or wild-type antigen 85B genes
| Time (min)a | 85B mRNA concnb
|
Synthetic/wild-type ratio | |
|---|---|---|---|
| Wild type | Synthetic | ||
| 0 | 1.0c | 0.8 | 0.8 |
| 30 | 5.1 | 12.7 | 2.5 |
| 60 | 6.2 | 12.2 | 2.0 |
| 300 | 7.0 | 12.1 | 1.7 |
Minutes postinduction of expression vector with IPTG.
Based on densitometry measurements.
Value set at 1.
