TABLE 2.
Fine specificities and avidities of MAbs
| MAb | Specificity by FELISA | Specificity by PPTa
|
Concn at 50% bindingb
|
||||
|---|---|---|---|---|---|---|---|
| MCPS | OAc− | K92 | MCPS | OAc− | K92 | ||
| MCPSg | |||||||
| IgM(κ) | |||||||
| 3624.22 | MCPS | + | −c | − | 0.01 | − | −d |
| 1863.5 | MCPS | − | − | − | 0.05 | − | − |
| 2010.10 | OAc− ≥ MCPS >> K92 | + | + | + | 0.025 | 0.03 | >3.0 |
| 1702.10 | OAc− >> MCPS | + | + | − | >3.0 | 0.2 | − |
| 1922.2 | OAc− >> MCPS | − | − | − | 2.0 | 0.08 | − |
| IgG3(κ) | |||||||
| 1705.18 | MCPS | + | − | − | 0.7 | − | − |
| 1846.13 | MCPS | + | − | − | 0.35 | − | − |
| 2055.5 | MCPS | + | − | − | 0.2 | − | − |
| 2750.27 | MCPS | + | − | − | >3.0 | − | − |
| 3006.18 | MCPS | + | − | − | 2.1 | − | − |
| 3079.6 | MCPS > OAc− | + | + | − | 0.3 | 0.8 | − |
| 181.1 | OAc− >>> MCPS | + | + | − | >3.0 | 0.002 | − |
| 2016.3 | OAc− >> MCPS | + | + | − | >3.0 | 0.1 | − |
| IgG1(κ) | |||||||
| 1946.13 | MCPS >>> OAc− | + | − | − | 0.001 | >3.0 | − |
| 177.16 | OAc− > MCPS | + | + | − | 0.03 | 0.004 | − |
| IgG2b(κ) | |||||||
| 78.2 | MCPS | − | − | − | >3.0 | − | − |
| C2 | |||||||
| IgA(κ) | |||||||
| C2/273.7 | OAc− >> MCPSe | − | − | − | 0.02 | 0.015 | − |
| C2/969.3 | OAc− >> MCPSe | + | + | − | 0.09 | 0.015 | − |
| IgG1(κ) | |||||||
| C2/205.10 | MCPS >>> OAc− | + | − | − | 0.003 | 1.0 | − |
| C2/951.8 | MCPS > OAc− | + | − | − | 0.006 | 0.05 | − |
| C2/630.10 | MCPS > OAc− | + | + | − | 0.003 | 0.03 | − |
| C2/735.4 | MCPS > OAc− | + | − | − | 0.005 | 0.09 | − |
| C2/35.3 | MCPS ≈ OAc− | + | + | − | 0.004 | 0.005 | − |
| C2/233.2 | MCPS ≈ OAc− | − | − | − | 0.15 | 0.5 | − |
| C2/256.8 | MCPS ≈ OAc− | + | + | − | 0.005 | 0.003 | − |
| C2/998.3 | MCPS ≈ OAc− | + | + | − | 0.003 | 0.003 | − |
| C2/974.1 | MCPS ≈ OAc− >>> K92 | + | + | −f | 0.002 | 0.003 | 5.0 |
| C2/1076.10 | MCPS ≈ OAc− >>> K92 | + | + | −f | 0.005 | 0.002 | 1.5 |
| C2/655.7 | OAc− >> MCPS | + | + | − | 0.3 | 0.003 | − |
| C2/181.7 | OAc− > MCPS | + | + | − | 0.1 | 0.01 | − |
| C2/706.12 | OAc− > MCPS >>> K92 | + | + | −f | 0.02 | 0.003 | 3.0 |
| CP | |||||||
| IgG1(κ) | |||||||
| CP1049.19 | MCPS ≈ OAc− | + | − | − | 0.25 | 0.12 | − |
| CP875.2h | MCPS ≈ OAc− | + | + | − | 0.005 | 0.0015 | − |
| CP882.2h | MCPS ≈ OAc− | − | − | − | 0.002 | 0.0015 | − |
| CP947.6h | MCPS ≈ OAc− | + | + | − | 0.007 | 0.002 | − |
| CP1092.23h | MCPS ≈ OAc− | + | + | − | 0.007 | 0.003 | − |
| CP1160.12h | MCPS ≈ OAc− | + | + | − | 0.007 | 0.003 | − |
| CP163.6h | OAc− >> MCPS | + | + | − | 0.55 | 0.0015 | − |
| IgG2b(κ) | |||||||
| CP1050.20 | MCPS > OAc− | + | − | − | 0.0025 | 0.015 | − |
Determined by Ouchterlony precipitation (PPT). Proteins were tested at 1 mg/ml.
Proteins were tested by 12 threefold serial dilutions starting at 3 μg/ml. Values are the concentrations at 50% binding in micrograms per milliliter determined at the midpoint of the linear part of the titration curve on PS-coated plates.
Not detectable at highest concentration tested (1 mg/ml).
Not detectable at highest concentration tested (3 μg/ml).
Determined by ascites fluid diluted 1:100.
Negative with the purified protein, but undiluted ascites was positive on all three Ags and negative on K1.
The fine specificity and precipitation values with MCPS and OAc− (for 15 of 16) and the concentrations at 50% binding (for 7 of 16) MCPS MAbs were previously published (51) and are reproduced with the permission of The Journal of Immunology.
These hybridomas were from mice boosted with fixed bacteria. All other CP MAbs came from mice boosted with purified MCPS.