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. 2022 Feb 12;32(23):5420–5437. doi: 10.1093/cercor/bhac024

Fig. 1.

Fig. 1

Microscopic images showing M2R-immunogold distribution in somatodendritic profiles contacted by CB1R-labeled axon terminals in the PL-PFC of vehicle-injected mice. a) M2R immunogold particles are localized to the cytoplasm along the outer nuclear envelope (ne) and nearby tubulovesicles (tv) in a neuronal soma contacted by an axon terminal containing CB1R-immunoperoxidase reaction product. M2R immunogold particles are also seen in nearby dendrites (M2-d1,2), one of which shows partially aggregated (circle) restricted immunoperoxidase reaction product possibly reflecting infrequently observed DAB precipitate. b) M2R-immunogold particles are distributed within the cytoplasm of a large dendrite at a distance from a CB1R-peroxidase labeled axon terminal and are also present on the presynaptic plasma membrane of an axon terminal (M2-t) forming an asymmetric synapse with an unlabeled dendritic spine (us). c) M2R immunogold has a cytoplasmic distribution in a large dendrite apposed by axon terminals that are unlabeled (white block arrows) or contain CB1R-immunoperoxidase reaction product but lack clearly defined synaptic membranes. d) Two transversely sectioned large dendrites showing cytoplasmic and plasmalemmal M2R-immunogold particles have apposed plasma membranes and contacts from a single axon terminal containing dense CB1R-immunoperoxidase labeling. e) A small dendrite containing exclusively cytoplasmic M2R immunogold is contacted by a CB1R-immunoperoxidase labeled terminal. White curved arrows in (a–e) indicate putative symmetric synapses between CB1R-labeled axon terminals (CB1-t) and M2R-immunogold labeled soma (M2-som) and dendrites (M2-d; M2-d1; M2-d2). White block arrows in (c) show appositions between unlabeled terminals (ut) and the M2R-labeled dendrite. Small straight arrows in (a–e) and black block arrows in (a and b), respectively, identify cytoplasmic and plasmalemmal M2R-immunogold particles in somatodendritic profiles. CB1-t = immunoperoxidase labeling for CB1R in axon terminal. M = mitochondrion. Scale bars = 500 nm.