Table 1.
Statistics of AqpZ4 association/dissociation kinetics and diffusion in C14, C16, C18 and C20 lipid bilayers
| τ1 (s) | τ2 (s) | DU (µm2/s) | CU (µm−2) | |
|---|---|---|---|---|
| C14 | 0.5 ± 0.14 (0.7) | 13 ± 3.3 (16 ± 4.9) | 0.42 ± 0.051 | 110 ± 39 |
| C16 | 0.7 ± 0.10 (0.7) | 9 ± 3.2 (8 ± 3.4) | 0.64 ± 0.039 | 30 ± 18 |
| C18 | 0.9 ± 0.10 (0.7) | 9 ± 1.1 (8 ± 1.7) | 0.59 ± 0.038 | 30 ± 10 |
| C20 | 0.6 ± 0.10 (0.7) | 15 ± 1.2 (17 ± 1.8) | 0.48 ± 0.031 | 160 ± 93 |
The time constants τ1 and τ2 were determined using Eq. (1). Brackets: Alternative fitting strategy: Fixing τ1 to 0.7 s and optimizing τ2. The 2D diffusion coefficients D2D and 2D concentration CU were determined using HS-AFM-HS. All statistics (mean ± s.e.) were determined from three biological replica in each condition.