Table 2.

Energies of membrane-mediated AqpZ interactions in C14, C16, C18 and C20 lipids

	ΔG0P-P(asso) (kBT)	ΔG0asso (kBT)	ΔG0P-P(diff) (kBT)	ΔG0diff (kBT)
C14	−6.9	−5.4 ± 0.41	−2.3	−3.2 ± 0.41
C16	−6.9	−6.8 ± 0.61	−2.3	−2.5 ± 0.27
C18	−6.9	−6.6 ± 0.34	−2.3	−2.3 ± 0.13
C20	−6.9	−5.1 ± 0.56	−2.3	−3.2 ± 0.25

The energies were determined based on the measured state dwell times from HS-AFM imaging and HS-AFM-HS as described in the text.