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. 2022 Dec 1;60(6):2200062. doi: 10.1183/13993003.00062-2022

FIGURE 5.

FIGURE 5

SLC1A5 mediates the hyperoxia-induced suppression of glutamine catabolism. a, b) Knockdown of SLC1A5 is confirmed at the a) mRNA and b) protein level in H1299 and A549 cells (n=5). c) Determination of the relative glutamine uptake in H1299 and A549 cells with shSLC1A5 treatment (n=5). d, e) The d) migration and e) invasion of H1299 cells (12 h) with short hairpin (sh)control and shSLC1A5 treatment (n=5); scale bar=50 μm. f) Representative bioluminescence images of mice 4 weeks after intravenous injection of H1299 cells. g) Lung tumour burden in mice 4 weeks after i.v. injection of H1299 cells and h) representative lung section (n=5). i) Photographs of Matrigel plugs excised from mice after 21 days of growth in vivo and j, k) quantitative analysis of the j) tumour volume and k) tumour weight (n=10). l) Detection of SLC1A5 expressions in xenograft models by Western blot analysis (n=6). *: p<0.05, **: p<0.01, ***: p<0.001 versus the indicated group; two-tailed t-test.