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. 2022 Nov 17;13:1034230. doi: 10.3389/fpls.2022.1034230

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Copyright © 2022 Li, Zhang, Fan, Wang, Wen, Mysore, Xie and Staehelin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of MtCHIT5b expression in M. truncatula nodules. (A) RT-qPCR analysis of MtCHIT5b transcript levels in nodules of M. truncatula R108 inoculated with S. meliloti Rm41 and harvested at 3 wpi. For comparison, MtCHIT5b expression was also analyzed in roots and leaves of these plants. Data indicate means ± SE of normalized values (mean value of nodules set to one) from three independent RNA extractions (n = 3) (Student’s t-test; ***, P< 0.001). (B-D) GUS staining analysis of R108 nodules expressing the MtCHIT5bp-GUS construct at 3 wpi (B) and 4 wpi (C, D). The picture in (D) shows a sectioned nodule. Bars = 200 μm in (B, D); 500 μm in (C).