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. 2022 Nov 17;13:1034230. doi: 10.3389/fpls.2022.1034230

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Copyright © 2022 Li, Zhang, Fan, Wang, Wen, Mysore, Xie and Staehelin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nod factor degradation in the rhizosphere of M. truncatula wild-type, chit5b and nfh1-3b mutant plants. NodSm-IV (C16:2, S) hydrolysis was analyzed in the rhizosphere of R108 wild-type, chit5b and nfh1-3b seedlings. Roots were pretreated with 0.5 μM NodSm-IV (C16:2, S) for 18 h and then assayed for 2 h with 5.4 μM NodSm-IV (C16:2, S). Formation of II (C16:2) was analyzed by reverse-phase HPLC. (A-C) Representative chromatograms showing NodSm-IV(C16:2, S) and II (C16:2) from wild-type (A), chit5b (B) and nfh1-3b (C) seedlings. Asterisks indicate nonidentified root compounds. (D) Hydrolytic activities deduced from HPLC chromatograms. Data indicate means ± SE (3 seedlings per sample; n = 3). The asterisks indicate significantly reduced cleavage activity in chit5b and nfh1-3b compared to wild-type plants (Student’s t test; **, P< 0.01; ***, P< 0.001).