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. 2022 Oct 31;298(12):102660. doi: 10.1016/j.jbc.2022.102660

Figure 5.

Figure 5

The RGG + CTD Δ62 is the key region of the ncRBD required for robust mRNA binding.A, Coomassie stain of recombinant proteins with C-terminal truncations to the ncRBD (RGG + CTD). The number of amino acids truncated from the CTD is indicated. B, EMSA of control reporter mRNA incubated with recombinant protein and stained with SYBR Green II. C, amino acid composition of overlapping regions of the ncRBD that are required for mRNA binding (RGG + CTD Δ62) and translational repression (RGG + CTD Δ54). CTD, C-terminal domain, ncRBD, noncanonical RNA-binding domain.