FIG. 3.
Comparison of the A. marginale-specific proliferative responses of antigen-draining (right) and adjuvant control (left) LNC from control and MSP-2-immunized calves. Cell suspensions were obtained from left and right prescapular LN biopsies of one calf per group at three different times. Freshly obtained (A, B, D, E, G, and H) or cryopreserved (C, F, and I) LNC from the antigen-draining right LN or adjuvant control left LN were assayed for proliferation against 0 to 25 μg of A. marginale or control URBC per ml (data not shown). LNC (3 × 105) were cultured for 4 days, radiolabeled, harvested, and counted. Data are the means ± the SEM of triplicate cultures and are representative of three separate assays performed with fresh or cryopreserved LNC.