TABLE 3.

A. marginale-specific IgG1 and IgG2 titers in the sera of calves immunized with MSP-2

Groupc and animal	Titer of subclassa:
	IgG1	IgG2
I
58	<100	<100
56	<100	<100
62	<100	<100
II
57	5,000	<100
63	1,000	<100
65	1,000	<100
III
59	10,000b	1,000
60	10,000b	<100
61	5,000b	<100

^aResults are presented for sera obtained 2 weeks after the fifth antigen inoculation. Sera were diluted 1:100 to 1:100,000 and tested for reactivity against A. marginale Florida homogenate on Western blots. The titer is defined as the reciprocal of the dilution giving a positive signal on the blots. A value of <100 indicates no reaction was observed at a dilution of 1:100 or higher. 

^bThe IgG1 titers in group III are significantly higher than the IgG1 titers in group II as determined by the one-tailed Student's t test (P = 0.025). 

^cAnimals in group I were immunized with IL-12, animals in group II were immunized with MSP-2, and animals in group III were immunized with MSP-2 and IL-12.