TABLE 4.
Composition of T-cell subsets in the lymph nodes of immunized calves
| Group and LNa | Percentage of LNCb | Percentage of CD3-positive T cellsc
|
||
|---|---|---|---|---|
| CD4 | CD8 | γ/δ TcR | ||
| I | ||||
| Left | 59.3 ± 10.0 | 50.0 ± 5.3 | 18.3 ± 4.5 | 23.0 ± 15.4 |
| Right | 59.7 ± 6.4 | 52.0 ± 1.7 | 17.3 ± 4.0 | 22.3 ± 12.2 |
| II | ||||
| Left | 64.6 ± 4.0 | 52.6 ± 9.3 | 22.0 ± 5.2 | 17.3 ± 3.0 |
| Right | 63.0 ± 16.5 | 54.6 ± 7.5 | 22.3 ± 3.8 | 14.0 ± 4.6 |
| III | ||||
| Left | 61.6 ± 17.8 | 53.3 ± 8.6 | 18.0 ± 2.6 | 19.3 ± 4.5 |
| Right | 49.0 ± 22.0 | 51.3 ± 5.1 | 20.6 ± 2.9 | 13.6 ± 9.8 |
Adjuvant control (left) or antigen-draining (right) LN were removed 3 days after the last inoculations of antigen and cell suspensions were prepared. Calves of group I were immunized with IL-12, calves of group II were immunized with MSP-2, and calves of group III were immunized with MSP-2 and IL-12.
Cells were stained with anti-CD3 MAb and analyzed by flow cytometry, and the results indicate the percentage of cells in the total LNC population that expressed CD3. Values are the means ± the SEM of the results for the three calves of each group.
Cells were stained with MAb against bovine CD4, CD8, or the γ/δ T-cell receptor and analyzed by flow cytometry. Values are the means ± the SEM for each group of calves.