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The GwAAP workflow
Schematic of the GwAAP workflow. A library of code peptides was established, and synthetic chromosomes were constructed using a synthetic biology technique. The N-terminus of genes on each chromosome contained 14-AA code peptides. The samples were lysed, and proteins were extracted and proteolyzed using trypsin. The peptide mixtures were further enriched by anti-HA antibodies and identified and quantified by LC-MS/MS in the DDA or PRM data acquisition modes.
