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. 2022 Nov 2;25(12):105471. doi: 10.1016/j.isci.2022.105471

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

GwAAP was applied to determine stoichiometry of the glycolysis and galactose metabolism pathways in yeast

(A) Workflow of the GwAAP analysis of S. cerevisiae grown in three different carbon sources.

(B) Examples of MS and MS/MS spectra of YPYDVPDYAGGASR (Gal80p) in two different carbon sources.

(C) The 26 proteins comprising glycolysis and galactose metabolism pathways were quantified. The colored bars with fold-change values indicate the relative abundance of proteins in the galactose- or glucose-containing medium groups (glucose group as control; red, upregulated; blue, downregulated). The number above the colored bars represents the protein stoichiometry, which is depicted as the log10 transformed abundance ratio of each protein versus the minimum protein.