REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Pierce™ Anti-HA Agarose	Thermo Scientific	Cat#26181; RRID: AB_2537081
Mouse mono-cloned α-HA	Sigma	Cat#H3663; RRID:AB_262051
Goat Anti-Mouse IgG (H&L)-HRP Conjugated	Easybio	Cat#BE0102-100; RRID:AB_2923205
Chemicals, peptides and recombinant proteins
Easytaq DNA polymerase	Transgen	Cat#AP111-01
Not I	NEB	Cat#R3189S
Urea	Sigma	Cat#U5128
Bradford protein assay kit	SanGon Biotech	Cat#C503041
Ammonium bicarbonate	Sigma-Aldrich	Cat#A6141
Fomic acid	Thermo Scientific	Cat#85171
Acetonitrile	Thermo Scientific	Cat#51101
10-plex TMT Label Reagents	Thermo Scientific	Cat#A34808
Trypsin	Promega	Cat#V5280
HPLC-grade H2O	J.T.Baker	Cat#4218-03
Critical commercial assays
Tanon™ High-sig ECL Western Blotting Substrate	Tanon	Cat#180-5001
Pierce™ Quantitative Fluorescent Peptide Assay	Thermo Scientific	Cat#23290
Deposited data
Mass spectrometry proteomics data	iProX (https://www.iprox.org.cn)	IPX0002656000
Experimental models: Organisms/strains
S. cerevisiae: Strain background: BY4741	EUROSCARF	http://www.euroscarf.de/index.php?name = News
Oligonucleotides
For all PCR primers, see Table S5	This paper	N/A
Recombinant DNA
For all plasmids, see Table S1	This paper	N/A
Software and algorithms
Thermo Proteome Discoverer 2.3	Thermo Scientific
Skyline (version 19.1.1.309)	Open Source	https://skyline.ms/project/home/software/skyline/begin.view
Xcalibur	Thermo Fisher Scientific	https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-software/multi-omics-data-analysis
GraphPad Prism8 (version 8.0.1)	Graphpad	GraphPad Prism; RRID: SCR_002798
R	R code	https://www.r-project.org
Other
Saccharomyces Genome Database (SGD)	SGD community	https://yeastgenome.org/