Figure 1.

Reduced pericyte coverage in Ccm1^ECKO mice

(A) Representative IF images of PECAM and PDGFRβ in the cerebella of Ccm1^ECKO and Ccm1^fl/fl mice at different time points. Cerebral cavernous malformations (CCMs) were enlarged vascular lesions (asterisks). The white box regions represented ECs covered by pericytes, whereas the purple box regions showed the ECs without pericyte coverage. Scale bar: 50 μm, 10 μm in higher-magnification images.

(B) Quantification of vascular area as shown in (A) at different time points.

(C) Quantification of the expression of PDGFRβ as shown in (A) at different time points.

(D and E) WB analysis showed that the level of PDGFRβ increased significantly in cerebellar microvessels of Ccm1^ECKO mice at P14. n = 3 or 4 mice per group. Data are presented as mean ± SD. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, one-way ANOVA with Tukey’s post-hoc test for B and C, unpaired Student’s t-test for E.