Figure 2.

Interruption of pericyte function exacerbated lesion burden in the cerebella and retinas of Ccm1^ECKO mice

(A and B) Stereomicroscopic images and hematoxylin-eosin (H&E) staining showed abundant CCM lesions in the cerebella of CP-673451-treated Ccm1^ECKO mice. Representative images of CCM lesions (asterisks) were shown in (B).

(C) Semiquantitative results of cerebellar CCM lesions as shown in (B).

(D) Representative confocal microscopy images of retina in Ccm1^fl/fl and Ccm1^ECKO mice treated with vehicle or CP-673451. The whole-mount staining of the mouse retina was performed with isolectin B4. The white dotted line marked CCM lesions.

(E) Large and complicated CCM lesion area in retinas of Ccm1^ECKO mice treated with CP-673451. n = 4 or 5 mice per group. Data are presented as mean ± SD. Scale bar: 1 mm in A; 200 μm in B; 500 μm in D. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗∗P < 0.001, unpaired Student’s t-test for upper panels of C and E, one-way ANOVA with Tukey’s post-hoc test for bottom panels of C.