Fig. 1.

The GARP complex is important for filamentation in response to diverse cues in C. albicans. a) Strains were grown in the presence or absence of 10 µM geldanamycin (GdA) for 7 h under shaking conditions prior to imaging. Scale bar represents 10 µm. b) Phenotype of GARP mutants grown in different filament-inducing media under static growth conditions. Strains were grown in the presence of the following inducing cues and conditions: Hsp90 inhibition with 10 μM geldanamycin (GdA) at 30°C for 6 h, cell cycle arrest with 40 μM nocodazole (NOC) at 30°C for 6 h, high temperature at 42°C for 5 h, Spider medium at 37°C for 5 h, RPMI medium at 37°C for 5 h, 5 mM N-acetyl-glucosamine (GlcNAc) at 37°C for 5 h, anaerobic growth at 37°C for 6 h, Lee’s medium at 37°C for 5 h, and 10% heat inactivated new born calf serum at 37°C for 5 h. Numbers correspond to degree of filamentation (DOF; see scale bar). Scale bar on microscopy image represents 10 µm. Heatmap was generated using the heatmap.2 function in R. c) Cells from overnight cultures were spotted onto filament inducing medium. Agar plates were incubated at 30°C (control), 37°C (serum, Spider, and GlcNAc) or 42°C (high temperature) for 3 days and imaged. d) J774A.1 macrophages were coincubated for 4 h with C. albicans strains, fixed with 4% formaldehyde, immunostained with a FITC anti-Candida antibody (green), and stained with DAPI to mark the macrophage nucleus (blue). Representative filaments are indicated with a white arrow. Scale bar represents 10 µm.