Fig. 2.

FRK promotes YAP ubiquitination and degradation. (A) Lysates of low passage primary GBM cell (GBM 1120D) transfected with Flag-YAP with or without FRK were examined by immunoblotting. (B&C) Representative immunoblots (B) and statistical results (C) of indicated proteins in FRK over-expression U251 and U87 GBM cells. (D-F) Lysates of FRK over-expression U251 and U87 GBM cells treated with 10 µg/ml cycloheximide (CHX) for the indicated times were examined by immunoblotting (D) and YAP levels were quantified (E, F). (G&H) Lysates of FRK over-expression U251 and U87 GBM cells treated with MG132 (20 μmol/L) or CQ (20 μmol/L) were immunoblotted as indicated antibodies. (I) HEK293T and U251 cells, as well as GBM 1120D cells were transfected with the indicated plasmids and treated with MG132. Cell lysates were immunoprecipitated using anti-YAP antibody and subjected to immunoblotting. *P < 0.05, **P < 0.01, ***P < 0.001.