Figure 1.

Effect of MKL1 on polyploidization of primary murine megakaryocytes. (A) Mean fluorescence intensity (MFI) of propidium iodide (PI) for megakaryocytes derived from wild-type (WT), Mkl1 kockout (KO), or double knockout (dKO) murine bone marrow, normalized to the MFI of the WT in each trial (dKO vs. WT: P=0.01, n=2). (B) MFI for megakaryocytes derived from WT, Mkl1 KO, or dKO murine bone marrow transduced with empty vector (EV) backbone or full-length (FL) MKL1 retrovirus. Each experiment was normalized to the MFI of the EV (WT: P=0.011, n=5; Mkl1 KO: P=0.007, n=6; dKO: P=0.028, n=4). (C) Representative ploidy peaks for WT, Mkl1 KO, and dKO marrow-derived megakaryocytes transduced with EV or FL MKL1 retroviruses, with cell number as a function of PI fluorescence. Far right: overlay of the mode-normalized ploidy peaks with EV (blue) and FL (red).