Figure 3.

Expression of GEF-H1 in response to wild-type and mutant forms of MKL1. Representative images of megakaryocyte progenitors transduced with MKL1 deletion constructs, cultured in thrombopoietin-only medium, and immunostained for α-tubulin and GEF-H1. Tubulin was detected with mouse anti-tubulin (1:250, ThermoFisher A11126) and AlexaFluor 488-labeled donkey anti-mouse AlexaFluor 488 (1:500, LifeTechnologies A21202). GEF-H1 was detected with rabbit anti-GEF-H1 (1:50, AbCam ab155785) and AlexaFluor 555-labeled donkey anti-rabbit (1:500, LifeTechnologies A31572). Hoechst 33342 was used to identify nuclei.