Figure 3.
Accelerated spreading of thymosin β4 knockout platelets. (A and B) Washed platelets were stimulated with 0.01 U mL1 thrombin and allowed to spread (5, 15, 30 minutes [min]) on fibrinogen (100 mg mL-1 ). DIC pictures were taken (A) and phase abundance was determined (B). Images are representatives of at least 6 animals per group. Scale bar: 3 mm. (C and D) Representative images of the platelet cytoskeleton ultrastructure of wild-type (WT) and Tmsb4x knockout (KO) mice on fibrinogen after 5 min (C) and 15 min (D). Scale bar: 500 nm.