Table 3. Linkage analysis of neutral sugars of the Epols extracted from ΔbcsB/pBerA and H111 WT pellicles produced in LB and NYG media.
Integration values of the peak areas were corrected by the effective carbon response factors (Sweet, Shapiro & Albersheim, 1975). Quantitation is reported as Molar Ratios Relative to 3-Man. Numbers next to sugars indicate the position of glycosidic linkages; t-Hex indicates terminal non-reducing hexose.
sugar | ΔbcsB/pBerA | H111 WT | ||
---|---|---|---|---|
LB | NYG | LB | NYG | |
2-Rha | 2.0 | 1.0 | 2.0 | 1.9 |
3-Rha | 1.6 | 0.6 | 1.4 | 1.3 |
t-Glc | - | - | - | 0.1 |
t-Man | - | 0.6 | - | 0.3 |
t-Gal | - | 1.1 | - | 0.4 |
3-Glc | 0.9 | 1.4 | - | 1.0 |
3-Man | 1.0 | 1.0 | 1.0 | 1.0 |
3-Gal | 0.6 | 1.5 | - | 0.6 |
3,6-Man | - | 0.1 | - | - |