Figure 4. Loss of RNF115 increases phagosomal proteolysis and enhances innate immune responses to intraphagosomal bacteria.

• A, B
  Intraphagosomal proteolysis and acidification assays show that loss of RNF115 increases phagosome maturation in BMA cells.
• C
  Complementing the RNF115 KO with WT RNF115 or a W259A mutant which is unable to bind to E2 enzymes, shows that RNF115 ligase activity is required for the increase of phagosome maturation. Shaded areas represent SEM.

Data information: six technical replicates; representative graphs of three independent experiments.