Table 1.
Yeast strains used in this study.
| Strain | Genotype | Source |
|---|---|---|
|
WT GFP‐Atg8 |
BY4741 (MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0) pRS316‐GFP‐Atg8 |
Brachmann et al (1998) |
|
opi3∆ GFP‐Atg8 |
BY4741 opi3∆::natMX4 pRS316‐GFP‐Atg8 |
This study |
|
cho2∆ GFP‐Atg8 |
BY4741 cho2∆::natMX4 pRS316‐GFP‐Atg8 |
This study |
|
WT Fba1‐GFP |
BY4741 FBA1‐yeGFP::hphNT1 | This study |
|
opi3∆ Fba1‐GFP |
BY4741 FBA1‐yeGFP::hphNT1 opi3∆::natMX4 | This study |
|
cho2∆ Fba1‐GFP |
BY4741 FBA1‐yeGFP::hphNT1 cho2∆::natMX4 | This study |
|
atg1∆ Fba1‐GFP |
BY4741 FBA1‐yeGFP::hphNT1 atg1∆::natMX4 | This study |
| WT (pho8Δ60 pho13Δ) | MATaleu2–3112 trp1 ura3‐52 pho8::pho8Δ60 pho13Δ::LEU2 | Noda et al (1995) |
| opi3∆ (pho8Δ60 pho13Δ) | MATaleu2–3112 trp1 ura3‐52 pho8::pho8Δ60 pho13Δ::LEU2 opi3∆::natMX4 | This study |
| cho2∆ (pho8Δ60 pho13Δ) | MATaleu2–3112 trp1 ura3‐52 pho8::pho8Δ60 pho13Δ::LEU2 cho2∆::natMX4 | This study |
| atg1∆ (pho8Δ60 pho13Δ) | MATaleu2–3112 trp1 ura3‐52 pho8::pho8Δ60 pho13Δ::LEU2 Atg1∆:: hphNT1 | This study |
| pep4∆ | BY4741 pep4∆::kanMX4 | This study |
|
atg1∆ GFP‐Atg8 |
BY4741 atg1∆::natMX4 pRS316‐GFP‐Atg8 |
This study |
|
opi3∆atg1∆ GFP‐Atg8 |
BY4741 atg1∆::natMX4 opi3∆::kanMX4 pRS316‐GFP‐Atg8 |
This study |
|
atg9∆ GFP‐Atg8 |
BY4741 atg9∆::natMX4 pRS316‐GFP‐Atg8 |
This study |
|
opi3∆atg9∆ GFP‐Atg8 |
BY4741 atg9∆::natMX4 opi3∆::kanMX4 pRS316‐GFP‐Atg8 |
This study |
|
ypt7∆ GFP‐Atg8 |
BY4741 ypt7∆::natMX4 pRS316‐GFP‐Atg8 |
This study |
|
opi3∆ ypt7∆ GFP‐Atg8 |
BY4741 ypt7∆::natMX4 opi3∆::kanMX4 pRS316‐GFP‐Atg8 |
This study |
|
WT Atg5‐mNG/ sfTq2‐Atg8 |
w303 ura3::pUAS_F_E_C_CORE1‐OsTIR1 trp1::pATG8‐sfTq2‐Atg8‐tATG8::hphNT1 ATG5‐mNG::tSYNTH8::CgTRP1 |
This study |
|
opi3∆ Atg5‐mNG/ sfTq2‐Atg8 |
w303 ura3::pUAS_F_E_C_CORE1‐OsTIR1 trp1::pATG8‐sfTq2‐Atg8‐tATG8::hphNT1 ATG5‐mNG::tSYNTH8::CgTRP1 opi3∆::CgHIS3 |
This study |
|
ymr1∆ Atg5‐mNG/ sfTq2‐Atg8 |
w303 ura3::pUAS_F_E_C_CORE1‐OsTIR1 trp1::pATG8‐sfTq2‐Atg8‐tATG8::hphNT1 ATG5‐mNG::tSYNTH8::CgTRP1 ymr1∆::KlURA3 |
This study |
|
ymr1∆ opi3∆ Atg5‐mNG/ sfTq2‐Atg8 |
w303 ura3::pUAS_F_E_C_CORE1‐OsTIR1 trp1::pATG8‐sfTq2‐Atg8‐tATG8::hphNT1 ATG5‐mNG::tSYNTH8::CgTRP1 opi3∆::CgHIS3 ymr1∆::KlURA3 |
This study |
|
WT Atg1‐mNG/ sfTq2‐Atg8 |
w303 ura3::pUAS_F_E_C_CORE1‐OsTIR1 trp1::pATG8‐sfTq2‐Atg8‐tATG8::hphNT1 ATG1‐mNG::tSYNTH8::CgTRP1 |
This study |
|
opi3∆ Atg1‐mNG/ sfTq2‐Atg8 |
w303 ura3::pUAS_F_E_C_CORE1‐OsTIR1 trp1::pATG8‐sfTq2‐Atg8‐tATG8::hphNT1 ATG1‐mNG::tSYNTH8::CgTRP1 opi3∆::CgHIS3 |
This study |
|
WT Atg9‐mNG/ sfTq2‐Atg8 |
w303 ura3::pUAS_F_E_C_CORE1‐OsTIR1 trp1::pATG8‐sfTq2‐Atg8‐tATG8::hphNT1 ATG9‐mNG::tSYNTH8::CgTRP1 |
This study |
|
opi3∆ Atg9‐mNG/ sfTq2‐Atg8 |
w303 ura3::pUAS_F_E_C_CORE1‐OsTIR1 trp1::pATG8‐sfTq2‐Atg8‐tATG8::hphNT1 ATG9‐mNG::tSYNTH8::CgTRP1 opi3∆::CgHIS3 |
This study |
|
WT mNG‐Atg8/mScarletI‐PXVam7 |
BY4741 trp1::pATG8‐mNeonGreen‐Atg8‐tATG8::hphNT1 vps38::NatNT2::pCUP1‐mScarletI‐Vam7(PX)‐tSYNTH13 |
This study |
|
opi3∆ mNG‐Atg8/ mScarletI‐PXVam7 |
BY4741 trp1::pATG8‐mNeonGreen‐Atg8‐tATG8::hphNT1 vps38::NatNT2::pCUP1‐mScarletI‐Vam7(PX)‐tSYNTH13 opi3::KlURA3 |
This study |
|
ymr1∆ mNG‐Atg8/mScarletI‐PXVam7 |
BY4741 trp1::pATG8‐mNeonGreen‐Atg8‐tATG8::hphNT1 vps38::NatNT2::pCUP1‐mScarletI‐Vam7(PX)‐tSYNTH13 ymr1::CgHIS3 |
This study |
|
opi3∆ymr1∆ mNG‐Atg8/ mScarletI‐PXVam7 |
BY4741 trp1::pATG8‐mNeonGreen‐Atg8‐tATG8::hphNT1 vps38::NatNT2::pCUP1‐mScarletI‐Vam7(PX)‐tSYNTH13 opi3::KlURA3 ymr1::kanMX4 |
This study |
|
ymr1∆atg1∆ mNG‐Atg8/ mScarletI‐PXVam7 |
BY4741 trp1::pATG8‐mNeonGreen‐Atg8‐tATG8::hphNT1 vps38::NatNT2::pCUP1‐mScarletI‐Vam7(PX)‐tSYNTH13 ymr1::kanMX4 atg1::CgHIS3 |
This study |
|
opi3∆ymr1∆atg1∆ mNG‐Atg8/ mScarletI‐PXVam7 |
BY4741 trp1::pATG8‐mNeonGreen‐Atg8‐tATG8::hphNT1 ape1::NatNT2::pCUP1‐mScarletI‐Ape1‐tAPE1 opi3::KlURA3 ymr1::kanMX4 atg1::CgHIS3 |
This study |
|
ymr1∆ mNG‐Atg8/ mScarletI‐Ape1 |
BY4741 trp1::pATG8‐mNeonGreen‐Atg8‐tATG8::hphNT1 ape1::NatNT2::pCUP1‐mScarletI‐Ape1‐tAPE1 ymr1::CgHIS3 |
This study |
|
opi3∆ymr1∆ mNG‐Atg8/ mScarletI‐Ape1 |
BY4741 trp1::pATG8‐mNeonGreen‐Atg8‐tATG8::hphNT1 ape1::NatNT2::pCUP1‐mScarletI‐Ape1‐tAPE1 opi3::KlURA3 ymr1::kanMX4 |
This study |
|
ymr1∆atg1∆ mNG‐Atg8/ mScarletI‐Ape1 |
BY4741 trp1::pATG8‐mNeonGreen‐Atg8‐tATG8::hphNT1 ape1::NatNT2::pCUP1‐mScarletI‐Ape1‐tAPE1 ymr1::kanMX4 atg1::CgHIS3 |
This study |
|
opi3∆ymr1∆atg1∆ mNG‐Atg8/ mScarletI‐Ape1 |
BY4741 trp1::pATG8‐mNeonGreen‐Atg8‐tATG8::hphNT1 ape1::NatNT2::pCUP1‐mScarletI‐Ape1‐tAPE1 opi3::KlURA3 ymr1::kanMX4 atg1::CgHIS3 |
This study |
|
atg3∆ Atg5‐mNG/ sfTq2‐Atg8 |
w303 ura3::pUAS_F_E_C_CORE1‐OsTIR1 trp1::pATG8‐sfTq2‐Atg8‐tATG8::hphNT1 ATG5‐mNG::tSYNTH8::CgTRP1 atg3∆::KlURA3 |
This study |
|
opi3∆atg3∆ Atg5‐mNG/ sfTq2‐Atg8 |
w303 ura3::pUAS_F_E_C_CORE1‐OsTIR1 trp1::pATG8‐sfTq2‐Atg8‐tATG8::hphNT1 ATG5‐mNG::tSYNTH8::CgTRP1 opi3∆::CgHIS3 atg3∆::KlURA3 |
This study |
|
WT BFP‐Ape1 |
BY4741 trp1::pATG8‐mNG‐Atg8‐tATG8::hphNT1 pDP105 (pRS315[pAPE1‐TagBFP‐Ape1‐tCYC1::pCUP1‐Ape1‐tAPE1]) |
This study |
|
opi3∆ BFP‐Ape1 |
BY4741 opi3::natMX4 trp1::pATG8‐mNG‐Atg8‐tATG8::hphNT1 pDP105 (pRS315[pAPE1‐TagBFP‐Ape1‐tCYC1::pCUP1‐Ape1‐tAPE1]) |
This study |