| Reagent/Resource | Reference or Source | Identifier or Catalog Number |
|---|---|---|
| Experimental Models | ||
| MC4100 cells (E. coli) | Casadaban (1997) | Prof. Dr. Genevaux, CBI Toulouse, France |
| Recombinant DNA | ||
| Genes (E. coli) | This study unless mentioned otherwise | Appendix Table S4 |
| Antibodies | ||
| Anti‐(pro)PhoA (Rabbit, monoclonal) | Chatzi et al (2017) (Ecolab/Davids) | 1/50,000 dilution |
| Anti‐rabbit (Peroxidase‐conjugated AffiniPure Goat) | Jackson ImmunoResearch Laboratories, Inc. | 111‐007‐003 (1/50,000 dilution) |
| Oligonucleotides and sequence‐based reagents | ||
| Custom oligos | Eurogentec | Appendix Table S2 |
| Chemicals, enzymes and other reagents | ||
| T4 DNA Ligase | Promega | M1801 |
| PFU Ultra Polymerase | Aligent | #600380 |
| Deuteriumoxide | Sigma Aldrich | P/N 151882 |
| Urea‐d4 | Sigma Aldrich | P/N 176087 |
| Formic Acid (MS grade) | Sigma Aldrich | F0507 |
| Acetonitrile (ACN, MS grade) | Merck Millipore | 100030 |
| Leucine Enkephalin (LeuEnk) | Waters | 186006013 |
| para‐Nitrophenolphosphate (PNPP) | Thermo Fisher Scientific | 34045 |
| Software | ||
| Canvas X | 2022 | https://canvasx.net |
| PyHDX | v0.3.3 (e8ea23e) | http://pyhdx.jhsmit.org |
| ImageJ | 1.53g 4 | https://imagej.nih.gov/ij/ |
| Jupyter Notebook (Anaconda, Python) | Python 3.6 | https://jupyter.org |
| AWSEM‐MD Frustratometer | Protein Frustratometer 2 (Parra et al, 2016) | http://frustratometer.qb.fcen.uba.ar |
| MassLynx | v4.1 (Waters) | Waters Corporation |
| ProteinLynx Global Server (PLGS) | v3.0.1 (Waters) | Waters Corporation |
| DynamX | v3.0 (Waters) | Waters Corporation |
| Clustal Omega | Sievers et al (2011) | https://www.ebi.ac.uk/Tools/msa/clustalo/ |
| PyMOL | v2.4 | https://pymol.org/2/ |
| Rosetta | 3.13 | https://www.rosettacommons.org/software |
| Other | ||
| Avanti J‐26S XPI, JLA 8.1000 rotor | Beckman | PN B10093AB |
| French Press | Thermo | FA‐078A + FA‐032 (40 k) Standard CELL |
| Sorvall RC 6 plus | Fisher Scientific | NB.81 |
| Ni2+‐NTA Agarose resin | Qiagen | ID: 30210 |
| Dialysis membranes (12–14 kDa MW cut‐off) | Medicell Membranes Ltd. | DTV.12000 |
| Plasmid DNA purification kit (NucleoSpin® Plasmid EasyPure) | Macherey‐Nagel | 740727.50. |
| Wizard SV Gel and PCR Clean‐Up System | Promega | A9281 |
| nanoACQUITY UPLC System with HDX Technology | Waters | Waters Corporation |
| Synapt G2 Mass Spectrometry instrument | Waters | Waters Corporation |
| MassPREP Micro Desalting column | Waters | 186004032 |
| Pepsin column | Sigma (pepsin) + Idex (cartridge) | P0609 + # 5051IP‐M07021‐005‐05TI |
| Nepenthesin‐2 | Affipro | AP‐PC‐004 |
| VanGuard C18 Pre‐column | Waters | 186003975 |
| C18 analytical column | Waters | 186002350 |
| SuperSignal™ West Pico PLUS Chemiluminescent Substrate | ThermoFisher Scientific | 34580 |
| ImageQuant LAS‐4000 (CCD‐camera system) | GE Healthcare Life Sciences | 28‐9610‐74 AC |
| Jasco J‐1500 | Jasco Inc. | J‐1000 series |
| Cary Eclipse Fluorescence Spectrophotometer | Agilent | Agilent Technologies |
| Nanodrop 2000 | Thermo | ND‐2000 |
| Vivaspin centrifugal concentrators (Vivaspin 500) | Viva products | VS0102+ |
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