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. 2022 Oct 18;41(23):e112338. doi: 10.15252/embj.2022112338

Figure EV3. Prion strain dependence of the 97 candidates.

Figure EV3

  1. Western Blot analysis of chronically 22L‐infected GT‐1/7 cells after PK treatment. Membrane is probed with anti‐PrP antibody POM1. P4, P5 and P6 corresponds to the amount of passaging after exposure to 22L brain homogenate. Brain homogenates were used as controls.
  2. Correlation of the effect of 97 candidates on their effect on PrPSc of two different prion strains (RML6 and 22L). Next to RML6 infected GT‐1/7, 97 hits were assessed for their effect on 22L prion infected GT‐1/7 for 72 h as well as 96 h‐long treatment duration. The high coefficient of determination (r 2‐value) of the effect observed for both prion strains indicates that the candidates do not show strain‐specificity.
  3. Gene set overrepresentation analysis for 161 hits and 40 hits from the prion modifier screen. No pathway passed the threshold for significance.

Source data are available online for this figure.