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Experimental setups. (A) Schematic of the protocol for human heart organoid fabrication. OCT was performed every day from day −1 to day 22, and every other day from day 24 to day 30. (B) Schematic of the OCT setup. Bottom left shows a 3D rendering of an hHO on day 27. Abbreviations: E8, Essential 8 Flex medium; RPMI, RPMI 1640 medium; CHIR, CHIR99021; BMP4, Recombinant Human Bone Morphogenetic Protein 4; and ActA, Activin A (see Materials and methods for details).
