FIG. 2.

Time kinetics of IL-8 secretion by stimulated TEC. A total of 10⁴ TEC/well were stimulated with IL-1α (1 ng/ml) in cell culture inserts over a period of 2 to 72 h. Secretion toward the upper and lower compartments was recorded. For each time point, supernatants of four wells were pooled and the IL-8 secretion was measured by ELISA (Fig. 2A). The functional activity of IL-8 production was determined by the neutrophil elastase release assay (Fig. 2B). The median of three experiments is shown. Data are given as nanograms of IL-8 protein (ELISA) and IL-8-like biological activity in nanogram IL-8 equivalents (release assay) contained in the total volume of supernatants.