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. 2022 Nov 23;49(1):14. doi: 10.3892/or.2022.8451

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Copyright: © Chee et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — HIF1α and HIF2α mRNA levels and HIF target genes change over time in hypoxia in PANC-1 cells. PANC-1 cells were treated with hypoxia (0.2% oxygen) or normoxia (21% oxygen) for 16 or 48 h. (A-C) RT-qPCR was conducted to determine (A) HIF1α, HIF2α, (B) CA9 and (C) Oct4 mRNA levels. Fold changes for RT-qPCR are shown relative to normoxic cells. Error bars represent the mean ± SD for n=9. The fold changes were analyzed using unpaired Student's t-test. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. HIF, hypoxia-inducible factor; RT-qPCR, reverse transcription-quantitative PCR; CA9, carbonic anhydrase IX; Oct4, octamer-binding transcription factor 4.