KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| RAD51AP1 | Mauro Modesti | #805 (Barroso-González et al., 2019; Modesti et al., 2007) |
| RAD51AP1 | Mauro Modesti | #806 (Barroso-González et al., 2019; Modesti et al., 2007) |
| RAD51 | Abcam | Cat. # ab133534; RRID:AB_272261 |
| TRF2 | Novus | Cat. #NB110–57130; RRID:AB_844199 |
| TRF1 | Jan Karlseder | N/A |
| FLAG (D6W5B) | Cell Signaling | Cat. # 14793S; RRID:AB_2572291 |
| FLAG (M2) | Millipore | Cat. # F1804; RRID:AB_262044 |
| γ-TUB | Sigma-Aldrich | Cat. # T6557; RRID:AB_477584 |
| PML (PG-M3) | Santa Cruz | Cat. # sc966; RRID:AB_628162 |
| Ubiquitin histone H2A (Lys119) | Millipore | Cat. # AB10029; RRID:AB_10617517 |
| c-Myc (9E10) | Abcam | Cat. # ab32; RRID:AB_303599 |
| RNAPII | Millipore | Cat. # 8WG16; RRID:AB_10013665 |
| PCNA | Abcam | Cat. # ab92552; RRID:AB_10561973 |
| S9.6 | Kerafast | Cat. # ENH001; RRID:AB_2687463 |
| BRG1 | Abcam | Cat. # ab4081; RRID:AB_304271 |
| PDS5a | ThermoFisher | Cat. # A300–089; RRID:AB_2162213 |
| ZMYND8 | ThermoFisher | Cat. # A302–090; RRID:AB_1604208 |
| SETX | ThermoFisher | Cat. # A301–104; RRID:AB_873128 |
| Critical commercial assays | ||
| ATMi (AZD0156) | Sellekchem | Cat. # S8375 |
| EZH2i (GSK126) | Sellekchem | Cat. # S7061 |
| 5,6-dichloro-1-β-D-ribofuranosyl-1H-benzimidazole (DRB) | Sigma | Cat. # D1916 |
| RO-3306 | Sigma-Aldrich | Cat. # SML0569 |
| Benzonase | Merck Millipore | Cat. # 70746 |
| 4-hydroxytamoxifen | Sigma | Cat. # H7904 |
| Doxycycline | Clontech | Cat. # 631311 |
| Shield Ligand | Clontech | Cat. # 632189 |
| Lipofectamine 2000 | Invitrogen | Cat. # 11668030 |
| Lipofectamine 3000 | Invitrogen | Cat. # L3000015 |
| Dharmafect Reagent 1 | Dharmacon | Cat. # T-2001 |
| Tel C PNA probe | PNA Bio | Cat. # F1004 |
| TERRA PNA Probe | IdT | (TAACCC)5-Alexa488 |
| ULTRA-hyb | ThermoFisher | Cat. # AM8663 |
| Duolink In Situ Detection Reagents | Sigma | Cat. # DUO-9214 |
| PDS5A targeting siRNA pool | Dharmacon | Cat. # L-014071 |
| BRG1 targeting siRNA pool | Dharmacon | Cat. # L-010431 |
| ZMYND8 targeting siRNA pool | Dharmacon | Cat. # L-017354 |
| Control non-targeting siRNA pool | Dharmacon | Cat. # D-001810 |
| RAD51AP1 targeting siRNA pool | Dharmacon | Cat. # L-017166 |
| SETX targeting siRNA pool | Dharmacon | Cat. # L-021420 |
| Deposited data | ||
| Proteomics Data | This study | ProteomeXchange Accession # PXD035943 |
| Raw data files | This study | Mendeley doi:10.17632/mbhnd2b9c7.1 |
| Experimental models: Cell lines | ||
| U2OS | ATCC | Cat# HTB-96; RRID:CVCL_0042 |
| HeLa LT | This study | N/A |
| GM847 | This study | N/A |
| LM216J | This study | N/A |
| U2OS TRF1-FokI WT/D450A | Roger Greenberg | (Dilley et al., 2016) |
| Oligonucleotides | ||
| Scramble antisense oligo (ASO) | IdT | cacgtctatacaccac (Chu et al., 2017) |
| TERRA ASO | IdT | taaccctaaccctaac (Chu et al., 2017) |
| RAD51AP15primeR | This study | ttcttatgtctcacaggccg |
| RAD51AP15primeF | This study | Gtgagacataagaaaccagtcaattactcaca gtt |
| RAD51AP13primerR | This study | caaaggtttaactcgtgcta |
| RAD51AP13primeF | This study | Gagttaaacctttgcatccaaatgccactagca cc |
| RAD51AP1 Isoform1Insert F | This study | tagtattccagctagtgcagtgccttgtacaaagatggctttagatgacaagctct |
| RAD51AP1 Isoform1Insert R | This study | ctagctggaatactaaaagtaccttcagggagcctttttttaggggttttctcttgt |
| Oligo-T | This study | Cy5-ttagggttagggttagggttagggttagggttagggttagg |
| Oligo-R-T | This study | cattgcatatttaaaacatgttggatctcaatttctattttatttttattttttatttctttattttaccatgaattcaaatgacctcttatcaagtgac |
| Oligo-R-B | This study | gtcacttgataagaggtcatttgaattcatcctaaccctaaccctaaccctaaccctaaccctaaccctaagagatccaacatgttttaaatatgcaatg |
| Oligo-D | This study | Cy5aaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcactatctcagcgatctgtctattt |
| TERRA RNA | This study | Cy5uuaggguuaggguuaggguuaggguuaggguuaggguuag |
| RNA-1 | This study | cugacaguuaccaaugcuuaaucagugagg |
| RNA-2 | This study | ccucacugauuaagcauugguaacugucag |
| RNA-3 | This study | uuauggcagcacugcauaauucucuuacug |
| RNA-4 | This study | gacggggagucaggcaacuauggaugaacg |
| RNA-5 | This study | cguucauccauaguugccugacuccccguc |
| RNA-6 | This study | aaaacuucauuuuuaauuuaaaaggaucua |
| RNA-7 | This study | uagauccuuuuaaauuaaaaaugaaguuuu |
| Recombinant DNA | ||
| BirA | O’Sullivan Lab | (García-Expósito et al., 2016) |
| BirA-TRF1 | O’Sullivan Lab | (García-Expósito et al., 2016) |
| BirA-RAD51AP1 | O’Sullivan Lab | This study |
| FLAG-RAD51AP1 K6K7 | O’Sullivan Lab | This study |
| GFP-RNaseH1-D210N | Lee Zou | (Nguyen et al., 2017) |
| GFP-RAD51AP1 | Mauro Modesti | (Modesti et al., 2007) |
| GFP-PDS5A | Jan Michael Peters | (Ladurner et al., 2016) |
| GFP-PDS5B | Jan Michael Peters | (Ladurner et al., 2016) |
| GFP-STAG1 | Jan Michael Peters | (Holzmann et al., 2019) |
| GFP-ZMYND8 | Kyle Miller | (Gong et al., 2015); (Addgene plasmid # 65401; RRID: Addgene_65401) |
| GFP-BRG1 | Kyle Miller | (Gong et al., 2015); (Addgene plasmid # 6539; RRID: Addgene_65391 |
| GFP-PBRM1 | Kyle Miller | (Gong et al., 2015); (Addgene plasmid # 65387; RRID: Addgene_65387 |
| GFP-KDM5A | Kyle Miller | (Gong et al., 2015); (Addgene plasmid # 65387; RRID: Addgene_65387 |
| GFP-NELF-E | Nabieh Ayoub | (Awwad et al., 2017) |
| GFP-SETX | Stephen West | (Yüce and West, 2013) |
| FLAG-RAD51AP1 | O’Sullivan Lab | (Barroso-González et al.,2019) |
| FLAG-RAD51AP1 SIM | O’Sullivan Lab | (Barroso-González et al.,2019) |
| FLAG-RAD51AP1 K269R | O’Sullivan Lab | (Barroso-González et al.,2019) |
| FLAG-RAD51AP11 ΔRAD51 | O’Sullivan Lab | (Barroso-González et al.,2019) |
| MBP-RAD51AP1-His | Sung lab | (Dunlop et al., 2012) |
| MBP-RAD51AP1 K6K7-His | Sung lab | (Dunlop et al., 2012) |
| RAD51 | Eric Greene Lab | (Xue et al., 2021) |