Table 2.

CRISPR/Cas systems for targeting proviral HIV-1 (In-vivo studies)

CRISPR/Cas system	Organism	Target region	Delivery	Results	References
SaCas9	Tg26 mice	LTR and viral genes	AAV9 (Adeno-associated vector)	Deletion at the target sequences in all the tissue samples studied. Excision of target sequence, reduction of 80–90% gag and env RNA	Kaminski et al. [48]
SaCas9	Tg26 mice NCr nude mouse BLT mice	LTR and viral genes	AAV (Adeno-associated vector)	Deletions at target sequences in samples collected from spleen, liver and bone-marrow. Excision and reduction of HIV gene expression	Yin et al. [59]
SpCas9	NRG rats	LTR and gag	Lentivirus	Cleavage and excision of integrated provirus in between the target sites	Bella et al. [60]
SaCas9	Humanized Mice (Engrafted with human CD34 + HSC)	LTR and gag	LASER ART therapy  +  AAV9 (Adeno-associated vector)	Effective decrease in viral load in dual treated mice compared to mice that were singularly treated with LASER ART or CRISPR alone In two of the seven mice, the viral load was undetectable	Dash et al. [62]
SaCas9	Rhesus macaques	LTR and gag	AAV9 (Adeno-associated vector)	Significant reduction of viral DNA in the blood and tissues. Precise cleavage and excision in samples collected from infected lymph nodes, spleen, bone-marrow and brain	Mancuso et al. [61]