. 2022 Nov 17;13:983954. doi: 10.3389/fphys.2022.983954

TABLE 4.

Effects of supplemental zinc source and zinc level on zinc concentration in different tissues of broilers.

Item	Added Zn (mg/kg)	Day 7			Day 14
Item	Added Zn (mg/kg)	Liver ^a (μg/g)	Tibia ^b (μg/g)	Pancreas ^a (μg/g)	Liver ^a (μg/g)	Tibia ^b (μg/g)	Pancreas ^a (μg/g)
Control ^c	0	20.55 ^d	176.3 ^d	32.76 ^d	8.91 ^d	137.3 ^d	24.14 ^d
ZnSO₄ ^c	40	26.51	333.1	61.44	23.88	361.0	45.31
	80	29.24	364.8	63.43	26.61	418.7	46.22
	120	34.43	394.3	76.10	24.67	426.9	57.75
Zn-Met ^c	40	26.62	375.0	61.61	24.09	372.8	48.29
	80	24.58	397.9	75.87	25.25	421.5	51.53
	120	31.48	403.3	71.65	28.02	432.7	51.21
Zn-Gly ^c	40	24.96	343.6	67.86	22.69	361.4	42.27
	80	25.42	394.4	67.86	26.78	415.2	54.13
	120	34.55	380.5	70.71	27.94	420.3	54.95
SEM		1.87	17.03	4.45	1.39	24.74	3.18
Zn-source ^e	ZnSO₄	30.06	364.0 ^b	66.99	25.05	402.2	49.76
	Zn-Met	27.56	392.1 ^a	69.71	25.79	409.0	50.34
	Zn-Gly	28.31	357.9 ^b	68.81	25.81	399.0	50.45
SEM		1.06	10.15	2.88	0.84	14.38	1.85
Added Zn ^e	40	26.03 ^b	350.5 ^c	63.64	23.55 ^b	365.0 ^b	45.29 ^b
(mg/kg)	80	26.41 ^b	370.7 ^b	69.05	26.21 ^a	418.5 ^a	50.63 ^ab
(mg/kg)	120	33.49 ^a	392.7 ^a	72.82	26.88 ^a	426.6 ^a	54.63 ^a
SEM		1.07	9.59	2.89	0.84	13.68	1.81
p-value
Zn-source		0.263	0.043	0.793	0.801	0.883	0.962
Added Zn		<0.001	0.012	0.099	0.017	0.006	0.002
Interaction		0.578	0.812	0.334	0.509	0.991	0.162
Linear ^f		<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

ZnSO₄, zinc sulfate heptahydrate; Zn-Met, zinc methionine chelate; Zn-Gly, zinc-glycine chelate; and SEM, standard error of mean.

^{^a}

Fresh tissue basis.

^{^b}

Ash weight basis.

^{^c}

Data are mean values of eight replicate pens per treatment.

^{^d}

Different from all Zn supplemental groups (p < 0.05).

^{^e}

Data are mean values of 24 replicate pens per treatment.

^{^f}

Linear effect of the added Zn level.

^a-c Means without the same superscript letters in the same column differ (p < 0.05).