Fig. 2.

YUMMER1.7 cells are more reliant on anaplerotic substrates in vitro than YUMM1.7 cells. A V_CS from [U-¹³C₆] glucose. B ¹³C enrichment of TCA cycle intermediates in cells incubated in [U-¹³C₆] glucose. C Relative V_CS in cells incubated in [U-¹³C₁₆] glutamine, normalized to data from cells incubated in [U-¹³C] glucose. D ¹³C enrichment of TCA cycle intermediates in cells incubated in [U-¹³C₆] glutamine. E Relative V_CS in cells incubated in [U-¹³C₁₆] palmitate. F ¹³C enrichment of TCA cycle intermediates in cells incubated in [U-¹³C₆] glucose. G–H YUMM1.7 and YUMMER1.7 cell number under conditions of substrate deprivation in vitro. The color of the asterisks denotes the group to which the group closest to the symbols was compared. I YUMM1.7 and YUMMER1.7 cell number under conditions of glutamine deprivation. J, K Effect of glucose and glutamine deprivation on the fraction live/dead cells, assessed by flow cytometry. L Impact of glucose or glutamine deprivation on cell number in five cell lines with varying immunogenicity. In all panels, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. The 2-tailed unpaired Student’s t test was used to compare two groups and ANOVA with Tukey’s multiple comparisons test to compare three or four groups