Fig. 3.

CXB enhanced autophagy in NPCs transfected with mRFP-GFP-LC3. A MOI titration assay for the optimal transfection. B Representative images and numbers of puncta of NPCs transfected with mRFP-GFP-LC3 for 6 h and replaced with fresh culture medium and subjected to IL-1β alone or with 2 µM or 10 µM CXB. Yellow puncta indicate autophagosome formation and were quantified (C). Each experiment was repeated three times. Scale bars for MOI titration, 200 μm. Scale Bars for autophagy, 30 μm