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. 2022 Dec 1;24:20. doi: 10.1186/s12575-022-00184-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Schematic workflow for untargeted urinary volatilome methodology. The workflow steps include: 1) Urine pretreatment, including collection and storage conditions, and matrix modifications to enhance the extraction of the volatile compounds; 2) Analytical conditions, including the selection and tuning of several parameters from extraction parameters, sample incubation, and analytical instrumentation; 3) Data analysis including normalization and identification sources. 4) Urine volatilome obtained after combining results of the 34 studies analysed. Created with BioRender.com