Table 1.
List of nuclear protein interactions detected for APC and CTNNB1 using AVA-Seq
| Protein1 | Protein2 | Orient1 | Orient2 | 2 mM | 5 mM | Log2FCmax | FDRmin | Library | Unique Pairs | APID |
|---|---|---|---|---|---|---|---|---|---|---|
| AXIN1 | APC | 227 | 64 | 159 | 132 | 8.820432722 | 0 | 2 | 201 | 1 |
| APC | AXIN2 | 30 | 122 | 77 | 75 | 7.520242693 | 2.10E−97 | 2 | 123 | 1 |
| CTNNB1 | CTBP1 | 2 | 0 | 1 | 1 | 4.103357711 | 2.20E−25 | 1 | 1 | 1 |
| CTNNB1 | TBL1XR1 | 1 | 0 | 0 | 1 | 1.968622264 | 3.41E−23 | 1 | 1 | 1 |
| CTBP1 | APC | 73 | 5 | 46 | 32 | 6.198931965 | 1.52E−21 | 2 | 67 | 1 |
| AXIN2 | CTNNB1 | 9 | 2 | 9 | 2 | 6.050628355 | 6.77E−20 | 2 | 9 | 1 |
| CREBBP | APC | 280 | 79 | 147 | 212 | 5.577882812 | 3.70E−17 | 2 | 307 | 1 |
| TCF7 | APC | 34 | 17 | 22 | 29 | 5.891080664 | 1.45E−14 | 2 | 41 | 0 |
| DVL3 | APC | 127 | 25 | 62 | 90 | 5.171919508 | 5.19E−11 | 2 | 125 | 0 |
| CTNNB1 | CTBP2 | 5 | 14 | 8 | 11 | 3.257456516 | 1.33E−10 | 2 | 19 | 1 |
| DVL1 | APC | 50 | 10 | 31 | 29 | 4.154109344 | 1.12E−09 | 2 | 55 | 1 |
| CTNNB1 | AXIN1 | 7 | 6 | 8 | 5 | 5.025473084 | 3.20E−09 | 2 | 12 | 1 |
| APC | JUN | 25 | 0 | 10 | 15 | 5.5769084 | 5.80E−05 | 2 | 19 | 0 |
| SOX17 | APC | 99 | 25 | 49 | 75 | 5.453437899 | 5.04E−09 | 2 | 87 | 0 |
| DVL2 | APC | 30 | 0 | 15 | 15 | 5.102993509 | 2.17E−07 | 2 | 26 | 1 |
| CTNNB1 | CREBBP | 12 | 2 | 8 | 6 | 3.038695645 | 6.17E−07 | 2 | 14 | 1 |
| CTNNB1 | SOX17 | 7 | 8 | 5 | 10 | 5.184004171 | 1.26E−05 | 2 | 12 | 1 |
| FOSL1 | APC | 13 | 0 | 6 | 7 | 3.353875751 | 6.85E−05 | 2 | 8 | 0 |
| DVL1 | CTNNB1 | 3 | 3 | 5 | 1 | 3.155612862 | 0.00041615 | 2 | 5 | 1 |
| DVL3 | CTNNB1 | 4 | 4 | 5 | 3 | 3.966139708 | 0.00105864 | 2 | 8 | 1 |
| TCF7 | CTNNB1 | 1 | 2 | 0 | 3 | 2.067171582 | 0.00728363 | 1 | 3 | 1 |
| CTNNB1 | CSNK1A1 | 1 | 0 | 1 | 0 | 3.500906308 | 0.00888600 | 1 | 1 | 1 |
| CTNNB1 | DVL2 | 2 | 0 | 1 | 1 | 4.682673739 | 0.00891623 | 1 | 1 | 1 |
Columns 1 and 2 (Protein 1, 2) list the protein pair tested. Orient 1 and 2, show how often the pair is detected in each orientation (Orient 1 is AD-associated; Orient 2 is DBD-associated); followed by a 3-AT condition to determine the number of pairs detected in 2 mM vs. 5 mM. Significant interactions filtered by Log2FCmax and FDRmin values. The number of libraries shows if the pairs are captured in a single library or both (with 2 being the maximum). The unique fragment pairs represent the number of unique fragments captured for each protein pair. The APID concludes if the pairs are novel (0) or known [1] previously from Agile Protein Interactomes DataServer (APID; [27])